Abstract

NUNEZ YEGROS, Olga Lorena et al. Detection and molecular characterization of Anaplasma marginale in DNA from blood samples of bovines from Paraguay. Investig. estud. UNA [online]. 2025, vol.16, n.2, pp.6-17. ISSN 2070-0415.  https://doi.org/10.18004/rdgic.investig.estud.una.2025.diciembre.3016025171.

Anaplasma marginale is an intraerythrocytic bacteria responsible for the disease known as bovine anaplasmosis, which has a great impact on livestock in tropical and subtropical regions. The importance of using conventional PCR (cPCR) is the early detection of persistent infections, since other study methods may present limitations when are diagnosed. The region of the Msp5 gene is specific for the detection of persistent infections. The present work was carried out using 86 bovine DNA samples, from whole blood using the cPCR technique for the amplification of the Msp5 gene. Thirty five percent (30/86) of the samples were positive for Anaplasma marginale. Of the positive samples, three were sequenced. This analysis determined the presence of A. marginale with an identity level of 99 - 100%. The construction of the phylogenetic tree showed that the samples obtained were closely related to other sequences previously reported in countries from Asia and Africa. This study shows prevalence of the disease in Paraguayan cattle. Furthermore, it is noted that this is the first molecular evidence of A. marginale in Paraguay done by molecular techniques, so it is recommended to expand studies on its pathogenicity, transmission mechanism and geographical distribution.

Keywords : DNA; Anaplasma marginale; cPCR; bovine; blood.

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