Resumen

ENCISO-MALDONADO, Guillermo Andrés et al. Evaluación del crecimiento y esporulación in vitro de Cercospora sp. y Septoria sp. en diferentes medios de cultivoEvaluation of in vitro growth and sporulation of Cercospora sp. and Septoria sp. in different culture media. Rep. cient. FACEN [online]. 2025, vol.16, n.1, pp.19-25. ISSN 2222-145X.  https://doi.org/10.18004/rcfacen.2025.16.1.019.

This study aimed to evaluate the in vitro mycelial growth and sporulation capacity of the phytopathogenic fungi Cercospora sp. and Septoria sp. (causal agents of Soybean Leaf Blight and Brown Spot, respectively) in different culture media: Leaf Decoction Agar (LDA), Grain Decoction Agar (GDA), and Potato Dextrose Agar (PDA). Mycelial growth was measured every three days for 15 days of incubation, and spore production was quantified at the end of the experiment using a Neubauer chamber. Significant differences in vegetative growth among the media were detected. For Cercospora sp., the PDA medium exhibited the highest growth from day 6 to day 15, reaching an average of 21.44 mm, significantly higher than GDA and LDA. In contrast, for Septoria sp., the GDA medium was the most effective, reaching an average growth of 8.84 mm by day 15, while PDA showed the lowest growth (3.65 mm). This study represents the first report on the use of the GDA medium for studying the in vitro growth of Septoria sp. The results demonstrated that none of the tested media induced sporulation under the evaluated conditions, with only sterile mycelial growth observed. It is concluded that, although the evaluated media supported mycelial growth, it is necessary to explore new environmental conditions and alternative media to optimize sporulation, given the relevance of these species in the epidemiology and management of soybean diseases.

Palabras clave : Phytopathogenic fungi; growth; in vitro; culture medium; soybean.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )