<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1812-9528</journal-id>
<journal-title><![CDATA[Memorias del Instituto de Investigaciones en Ciencias de la Salud]]></journal-title>
<abbrev-journal-title><![CDATA[Mem. Inst. Investig. Cienc. Salud]]></abbrev-journal-title>
<issn>1812-9528</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Investigaciones en Ciencias de la Salud]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1812-95282017000300089</article-id>
<article-id pub-id-type="doi">10.18004/mem.iics/1812-9528/2017.015(03)89-092</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Identificación molecular del sexo en 9 especies de aves del Centro de Investigación en Animales Silvestres de la hidroeléctrica de ITAIPU, lado paraguayo]]></article-title>
<article-title xml:lang="en"><![CDATA[Sex identification by molecular methods in 9 species of birds from the wild animal research center of ITAIPU dam on the paraguayan side]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[del Puerto]]></surname>
<given-names><![CDATA[Florencia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pésole]]></surname>
<given-names><![CDATA[Diana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Molina]]></surname>
<given-names><![CDATA[Santiago]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vera]]></surname>
<given-names><![CDATA[Karen]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Arias]]></surname>
<given-names><![CDATA[Marcela]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sosa]]></surname>
<given-names><![CDATA[Javier]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ortiz]]></surname>
<given-names><![CDATA[María Luisa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Fernández]]></surname>
<given-names><![CDATA[José]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Garay]]></surname>
<given-names><![CDATA[Anastacio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional de Asunción Instituto de Investigaciones en Ciencias de la Salud Departamento de Medicina Tropical]]></institution>
<addr-line><![CDATA[San Lorenzo ]]></addr-line>
<country>Paraguay</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,ITAIPU BINACIONAL Centro de Investigación de Animales Silvestres de ITAIPU ]]></institution>
<addr-line><![CDATA[Hernandarias ]]></addr-line>
<country>Paraguay</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2017</year>
</pub-date>
<volume>15</volume>
<numero>3</numero>
<fpage>89</fpage>
<lpage>92</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_arttext&amp;pid=S1812-95282017000300089&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_abstract&amp;pid=S1812-95282017000300089&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_pdf&amp;pid=S1812-95282017000300089&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN La importancia de determinar el sexo en especies monomórficas que viven en cautiverio conlleva a estrategias de apareamiento a fin de poder incentivar la reproducción. Existen métodos no moleculares de reconocimiento pero son factibles en edad adulta y mucho más difícil cuando son polluelos. Mediante la técnica molecular de PCR, se amplificó el gen de la Cromo Helicasa de Unión al ADN empleando los cebadores, 2550F/2718R. Por este método molecular se consiguió determinar el sexo en 19 de 23 especies de las cuales 3 son ejemplares de: Amazona aestiva, 1 de Pipile grayi, 1 de Bubo virginianus, 4 de Ara chloropterus, 6 de Crax fasciolata, 2 de Caracara plancus, 3 de Chauna torquata, 1 de Cariama cristata y 2 de Cairina moschata del Centro de Investigación de Animales Silvestres de ITAIPU binacional, del lado paraguayo. Nuestros resultados sugieren que el par de cebadores 2550F/2718R podría ser de utilidad para determinar el sexo en las especies estudiadas en el país.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT In monomorphic species living in captivity, determining the sex is important because it may allow choosing mating strategies to enhance reproduction. Sex can be determined either by non-molecular recognition methods, which are only feasible in adult birds, or by molecular techniques, which may allow sexing monomorphic species at young ages. Thus, in this study, we used molecular techniques such PCR to amplifying the CHD gene by using 2550F/2718R primers. This method allowed us to determine the sex in 19 out of 23 bird specimens from the Centro de Investigación de Animales Silvestres (CIASI) of the ITAIPÚ BINACIONAL at Paraguayan side. The specimens belonged to the following species: Amazona aestiva (3), Pipile grayi (1), Bubo virginianus (1), Ara chloropterus (4), Crax fasciolata (6), Caracara plancus (2), Chauna torquata (3), Cariama cristata (1) and Cairina moschata (2). The 2550F/2718R primers were useful for determine the sex of the studied species.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Sexado]]></kwd>
<kwd lng="es"><![CDATA[aves]]></kwd>
<kwd lng="es"><![CDATA[2550F/2718R]]></kwd>
<kwd lng="es"><![CDATA[P2/P8]]></kwd>
<kwd lng="es"><![CDATA[Paraguay]]></kwd>
<kwd lng="en"><![CDATA[Sexing]]></kwd>
<kwd lng="en"><![CDATA[birds]]></kwd>
<kwd lng="en"><![CDATA[2550F/2718R]]></kwd>
<kwd lng="en"><![CDATA[Paraguay]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Molecular techniques for sex identification in birds]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Dubiec]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Zagalska-Neubauer]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<source><![CDATA[Biological Lett]]></source>
<year>2006</year>
<volume>43</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>3-12</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Sex identification in birds using two CHD genes]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Griffiths]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Daan]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Dijkstra]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
</person-group>
<source><![CDATA[Proceedings of the Royal Society B]]></source>
<year>1996</year>
<volume>263</volume>
<page-range>1251-6</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[A DNA test to sex most birds]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Griffiths]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Double]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Orr]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Dawson]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular Ecology]]></source>
<year>1998</year>
<volume>7</volume>
<page-range>1071-5</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[First gene on the avian W chromosome (CHD) provides a tag for universal sexing on non-ratite birds]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ellegren]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
</person-group>
<source><![CDATA[Proceedings of the Royal Society B]]></source>
<year>1996</year>
<volume>263</volume>
<page-range>1635-41</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Feasibility of Non-Invasive Molecular Method for Sexing of Parrots]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bosnjak]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Stevanov-Pavlovic]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Vucicevic]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Stevanovic]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Simeunovic]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Resanovic]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
</person-group>
<source><![CDATA[Pakistan Journal of Zoology]]></source>
<year>2013</year>
<volume>45</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>715-20</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bailey]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Capture and Handling]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Samour]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<source><![CDATA[Avian Medicine]]></source>
<year>2008</year>
<edition>2da</edition>
<page-range>1-2</page-range><publisher-loc><![CDATA[Canada ]]></publisher-loc>
<publisher-name><![CDATA[Elsevier]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Campbell]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Hematology]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Ritchie]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Harrison]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Harrison]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
</person-group>
<source><![CDATA[Avian Medicine: Principles and application]]></source>
<year>1994</year>
<page-range>176-98</page-range><publisher-name><![CDATA[Wingers Publishing Inc]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Usefulness of seminested multiplex PCR in surveillance of imported malaria in Spain]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rubio]]></surname>
<given-names><![CDATA[JM]]></given-names>
</name>
<name>
<surname><![CDATA[Benito]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Berzosa]]></surname>
<given-names><![CDATA[PJ]]></given-names>
</name>
<name>
<surname><![CDATA[Roche]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Puente]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Subirats]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<source><![CDATA[Journal of Clinical Microbiology]]></source>
<year>1999</year>
<volume>37</volume>
<numero>10</numero>
<issue>10</issue>
<page-range>3260-4</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[A simple and universal method for molecular sexing of nonratite birds]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Fridolfsson]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Ellegren]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
</person-group>
<source><![CDATA[Journal of Avian Biology]]></source>
<year>1999</year>
<volume>30</volume>
<page-range>116-21</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
