<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2709-0817</journal-id>
<journal-title><![CDATA[Revista investigaciones y estudios-UNA]]></journal-title>
<abbrev-journal-title><![CDATA[Investig. estud. UNA]]></abbrev-journal-title>
<issn>2709-0817</issn>
<publisher>
<publisher-name><![CDATA[Dirección General de Investigación Científica y Tecnológica-UNA]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2709-08172024000200051</article-id>
<article-id pub-id-type="doi">10.57201/ieuna2424618</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Optimización de la metodología para la cuantificación de Cannabidiol y Tetrahidrocannabinol mediante Cromatografía Líquida de Alta Resolución]]></article-title>
<article-title xml:lang="en"><![CDATA[Optimization of the quantification of Cannabidiol and Tetrahydrocannabinol methodology by High Performance Liquid Chromatography]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ayala]]></surname>
<given-names><![CDATA[José F.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Nakayama]]></surname>
<given-names><![CDATA[Héctor D.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Samudio Oggero]]></surname>
<given-names><![CDATA[Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Benítez]]></surname>
<given-names><![CDATA[Emilio F.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ries]]></surname>
<given-names><![CDATA[Andreas]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional de Asunción Centro Multidisciplinario de Investigaciones Tecnológicas ]]></institution>
<addr-line><![CDATA[San Lorenzo ]]></addr-line>
<country>Paraguay</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Nacional de Asunción. Facultad de Ciencias Químicas Carrera de Bioquímica]]></institution>
<addr-line><![CDATA[San Lorenzo ]]></addr-line>
<country>Paraguay</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad Nacional de Asunción Facultad Politécnica Laboratorio de Bio-materiales]]></institution>
<addr-line><![CDATA[San Lorenzo ]]></addr-line>
<country>Paraguay</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2024</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2024</year>
</pub-date>
<volume>15</volume>
<numero>2</numero>
<fpage>51</fpage>
<lpage>65</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_arttext&amp;pid=S2709-08172024000200051&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_abstract&amp;pid=S2709-08172024000200051&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_pdf&amp;pid=S2709-08172024000200051&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Los cannabinoides son compuestos terpenfenólicos obtenidos de la especie Cannabis sativa (Cannabaceae), que se distribuye por todo el mundo en múltiples variedades. La concentración de cada cannabinoide en una planta de cannabis está estrechamente relacionada con la variedad. Hay ejemplares en los que se encuentran mayores rendimientos de un cannabinoide concreto que del resto de cannabinoides. Organismos internacionales como la Oficina de las Naciones Unidas contra la Droga y el Delito recomiendan la aplicación de la Cromatografía Líquida de Alta Resolución como método analítico para la cuantificación de cannabinoides. En el presente trabajo se han evaluado las diferentes variables que influyen en un análisis cromatográfico como la temperatura, el pH de la muestra, el flujo de elución y la longitud de onda y se han estandarizado las condiciones cromatográficas para la cuantificación analítica de los metabolitos de cannabidiol y tetrahidrocannabinol.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Cannabinoids are terpenophenolic compounds obtained from the species Cannabis sativa (Cannabaceae), which is worldwide distributed across multiple varieties. The concentration of each cannabinoid in a cannabis plant is closely related to the variety. There are specimens in which higher yields of one specific cannabinoid are found than the remaining cannabinoids. International organizations such as the United Nations Office on Drugs and Crime, recommend the application of High-Performance Liquid Chromatography as an analytical method for the quantification of cannabinoids. In the present work, the different variables that influence a chromatographic analysis such as temperature, sample pH, elution flow and wavelength have been evaluated and the chromatographic conditions for the analytical quantification of the metabolites cannabidiol and tetrahydrocannabinol have been standardized.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Cannabis sativa]]></kwd>
<kwd lng="en"><![CDATA[industrial hemp]]></kwd>
<kwd lng="en"><![CDATA[HPLC]]></kwd>
<kwd lng="en"><![CDATA[Medical Cannabis.]]></kwd>
<kwd lng="es"><![CDATA[Cannabis sativa]]></kwd>
<kwd lng="es"><![CDATA[cáñamo industrial]]></kwd>
<kwd lng="es"><![CDATA[HPLC]]></kwd>
<kwd lng="es"><![CDATA[Cannabis medicinal.]]></kwd>
</kwd-group>
</article-meta>
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