<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2664-2891</journal-id>
<journal-title><![CDATA[Revista científica ciencias de la salud]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. cient. cienc. salud]]></abbrev-journal-title>
<issn>2664-2891</issn>
<publisher>
<publisher-name><![CDATA[Universidad del Pacífico]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2664-28912019000100046</article-id>
<article-id pub-id-type="doi">10.53732/rccsalud/01.01.2019.46</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Criopreservación de la cepa RH de Toxoplasma gondii utilizando como criopreservante DMSO y glicerol]]></article-title>
<article-title xml:lang="en"><![CDATA[Cryopreservation of the RH strain of Toxoplasma gondii using as cryopreservant DMSO and glycerol]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ozuna]]></surname>
<given-names><![CDATA[Carmen]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas]]></surname>
<given-names><![CDATA[Alejandra]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Arias]]></surname>
<given-names><![CDATA[Laura]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Meza,]]></surname>
<given-names><![CDATA[Teresa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guillén,]]></surname>
<given-names><![CDATA[Yvalena]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Acosta]]></surname>
<given-names><![CDATA[María Eugenia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional de Asunción Instituto de investigaciones en Ciencias de la Salud Departamento de producción]]></institution>
<addr-line><![CDATA[Asunción ]]></addr-line>
<country>Paraguay</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<volume>1</volume>
<numero>1</numero>
<fpage>46</fpage>
<lpage>51</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_arttext&amp;pid=S2664-28912019000100046&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_abstract&amp;pid=S2664-28912019000100046&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_pdf&amp;pid=S2664-28912019000100046&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[R E S U M E N El trabajo tuvo como objetivo evaluar la viabilidad de parásitos de Toxoplasma gondii de la cepa RH criopreservados en nitrógeno líquido durante 1 y 5 meses con dos criopreservantes: el glicerol 10% y dimetilsulfóxido (DMSO) 1,25%, 12,5% y 25%, para contar con un método eficaz de almacenamiento de taquizoitos por largos periodos de tiempo. Fueron descongelados e inoculados a un ratón por cada concentración de criopreservante. El control utilizado fueron taquizoitos mantenidos en ratones albinos. El número de parásitos al inicio fue menor al control y esto fue aumentando en los sucesivos pasajes. Tanto el Glicerol como el DMSO presentaron comportamientos semejantes, sin embargo, con el DMSO en concentraciones de 12.5 y 25% se observó mayor cantidad de parásitos/mL en los meses evaluados. Las diferentes concentraciones ensayadas resultan efectivas y no alteran la viabilidad de los parásitos de T gondii cepa RH criopreservados durante 1 y 5 meses.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[A B S T R A C T The objective of the work was to evaluate the viability of Toxoplasma gondii parasites of the RH strain cryopreserved in liquid nitrogen for 1 and 5 months with two cryopreservants: glycerol 10% and dimethylsulfoxide (DMSO) 1.25%, 12.5% and 25%. %, to have an effective method of storage of tachyzoites for long periods of time. One mouse was thawed and inoculated for each concentration of cryopreservant. The control used were tachyzoites maintained in albino mice. The number of parasites at the beginning was less than control and this was increasing in the successive passages. Both Glycerol and DMSO presented similar behaviors, however with DMSO at concentrations of 12.5 and 25%, a greater number of parasites / mL was observed in the months evaluated. The different concentrations tested are effective and do not alter the viability of parasites of T. gondii strain RH cryopreserved for 1 and 5 months.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[toxoplasma]]></kwd>
<kwd lng="es"><![CDATA[criopreservación]]></kwd>
<kwd lng="es"><![CDATA[dimetilsulfóxido]]></kwd>
<kwd lng="es"><![CDATA[glicerol]]></kwd>
<kwd lng="en"><![CDATA[toxoplasma]]></kwd>
<kwd lng="en"><![CDATA[cryopreservation]]></kwd>
<kwd lng="en"><![CDATA[dimethyl sulfoxide]]></kwd>
<kwd lng="en"><![CDATA[glycerol]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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