<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1812-9528</journal-id>
<journal-title><![CDATA[Memorias del Instituto de Investigaciones en Ciencias de la Salud]]></journal-title>
<abbrev-journal-title><![CDATA[Mem. Inst. Investig. Cienc. Salud]]></abbrev-journal-title>
<issn>1812-9528</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Investigaciones en Ciencias de la Salud]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1812-95282018000100033</article-id>
<article-id pub-id-type="doi">10.18004/mem.iics/1812-9528/2018.016(01)33-038</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Portación de fimH en aislados de Escherichia coli productor de Toxina Shiga provenientes de ganado bovino, Departamento Cordillera, Paraguay]]></article-title>
<article-title xml:lang="en"><![CDATA[Portability of fimH in isolates of Shiga toxin-producing Escherichia coli from cattle, Department of Cordillera, Paraguay]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Florentín]]></surname>
<given-names><![CDATA[Melisa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Acuña]]></surname>
<given-names><![CDATA[Patricia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas]]></surname>
<given-names><![CDATA[Natalia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez]]></surname>
<given-names><![CDATA[Fátima]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guillén]]></surname>
<given-names><![CDATA[Rosa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional de Asunción Instituto de Investigaciones en Ciencias de la Salud Departamento de Microbiología]]></institution>
<addr-line><![CDATA[San Lorenzo ]]></addr-line>
<country>Paraguay</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2018</year>
</pub-date>
<volume>16</volume>
<numero>1</numero>
<fpage>33</fpage>
<lpage>38</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_arttext&amp;pid=S1812-95282018000100033&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_abstract&amp;pid=S1812-95282018000100033&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iics.una.py/scielo.php?script=sci_pdf&amp;pid=S1812-95282018000100033&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Ciertas cepas de Escherichia coli productoras de toxina Shiga (STEC) tienen la capacidad de formar biofilm en alimentos y otras superficies, aumentando su potencial como fuente de contaminación. El gen fimH se ha asociado a la capacidad de formación de biofilm en E.coli. Este estudio observacional descriptivo de corte transverso se realizó con el objetivo de describir la portación de fimH en aislados STEC provenientes de muestras de materia fecal de ganado bovino del Departamento de Cordillera en el 2016. Los aislados de STEC se obtuvieron por cultivos, extracción de ADN y amplificación por PCR de genes stx1 y stx2. El gen fimH se detectó por PCR convencional. Un total de 1006 aislamientos de E. coli se sometieron a extracción de ADN y amplificación por PCR para genes stx1 y stx2. De éstos, 269 se identificaron como STEC, en los que la detección del gen fimH se realizó por PCR convencional. Un producto de PCR representativo se sometió a secuenciación del gen fimH y mostró 100% de homología con secuencias de la Base de Datos de GenBank. De 269 aislamientos STEC, 129 aislamientos (48%) resultaron ser portadores del gen fimH y por tanto con potencial de formar biofilm. Esta frecuencia elevada representa un riesgo de persistencia de estos patógenos en elementos y superficies de trabajo de sitios de expendio y manipulación de productos cárnicos. Este trabajo contribuye como una herramienta esencial para seguir con la línea de investigación, obteniendo datos de suma importancia que ayuden a describir la situación de riesgo de contaminación alimentaria en que se encuentra el país.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Certain strains of Shiga toxin-producing Escherichia coli (STEC) have the ability to form biofilm in food and other surfaces, increasing their potential as a source of contamination. The fimH gene has been associated with the capacity for biofilm formation in E. coli. This cross-sectional descriptive observational study was carried out with the aim of describing the carrying of fimH in STEC isolates from faecal samples of bovine cattle from the Department of Cordillera in 2016. STEC isolates were obtained by cultures, extraction of DNA and PCR amplification of stx1 and stx2 genes. The fimH gene was detected by conventional PCR. A total of 1006 isolates of E.coli were subjected to DNA extraction and PCR amplification for stx1 and stx2 genes. Of these, 269 were identified as STEC, in which the detection of the fimH gene was carried out by conventional PCR. A representative PCR product was subjected to sequencing of the fimH gene and showed 100% homology with sequences from the GenBank Database. Of 269 STEC isolates, 129 isolates (48%) were found to be carriers of the fimH gene and therefore with the potential to form biofilm. This high frequency represents a risk of persistence of these pathogens in elements and work surfaces of meat products sale and handling sites. This work contributes as an essential tool to continue with the line of research, obtaining data of great importance that help to describe the situation of food contamination risk of the country.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[STEC]]></kwd>
<kwd lng="es"><![CDATA[biofilm]]></kwd>
<kwd lng="es"><![CDATA[ganado bovino]]></kwd>
<kwd lng="en"><![CDATA[STEC]]></kwd>
<kwd lng="en"><![CDATA[biofilm]]></kwd>
<kwd lng="en"><![CDATA[cattle.]]></kwd>
</kwd-group>
</article-meta>
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