SciELO - Scientific Electronic Library Online

 
vol.23 issue1Chemical control of Fusarium head blight of wheat and its effect on grain production and seed qualityAgronomic evaluation of nitrogen-fixing bacteria isolated from Andean soils in lettuce and tomato seedlings author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Investigación Agraria

On-line version ISSN 2305-0683

Abstract

OLIVEIRA BORGES COSTA NEVES, Flávia de et al. Culture media and effect of melaleuca essential oil in Stemphylium sp. micelial growth. Investig. Agrar. [online]. 2021, vol.23, n.1, pp.40-46. ISSN 2305-0683.  https://doi.org/10.18004/investig.agrar.2021.junio.2301578.

Species of Stemphylium genus are responsible for many plant diseases, as solanaceas in which stemphylium leaf blight, which productivity of these crops can be negatively affected due lacking in available fungicides. Therefore, the purpose of this work was to determine a culture medium that would allow of Stemphylium sp. mycelial growth isolated from onion for a later evaluation of melaleuca oil (M. alternifolia) effect under different in vitro concentrations. For this, in the first test eight culture media were tested: Potato-Dextrose-Agar (PDA); Juice V-8® 5% Agar; V-8® Juice 10% Agar; Juice V-8® Agar 20%; Tomato Juice 5% (ST5%); Tomato Juice 10% (ST10%); Tomato Juice 20% (ST20%) and Malt Extract Agar 2%. At ten days and fifteen days, the highest growth was identified with treatments ST20% and PDA and, conversely, treatments V8-5% and V8-10% the lowest growth. In the second trial, melaleuca oil was tested in five concentrations (0; 0, 2; 0,4; 0,6 and 0,8%) diluted in PDA and the MGR (mycelial growth rate) was determined. The melaleuca essential oil reduced the Stemphylium sp. mycelial growth with better fit to the quadratic equation y=28,074x2 - 46,136x + 19,94. The MGR was reduced by the oil added to culture medium and could represent a promising alternative for this pathogen control.

Keywords : In vitro control; Melaleuca alternifolia; Stemphylium leaf blight.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf )