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Investigación Agraria

On-line version ISSN 2305-0683


MORO STEFANEL, Charlene; SILVEIRA REINIGER, Lia Rejane; LEMOS SERROTE, Caetano Miguel  and  DA FONSECA ZIEGLER, Ana Cristina. Use of antibiotic streptomycin sulphate to the control in vitro contamination of nodal segments of Eugenia involucrate. Investig. Agrar. [online]. 2021, vol.23, n.1, pp.1-7. ISSN 2305-0683.

Decontamination of explants is a requirement for micropropagation efficiency. The aim of this work was to evaluate the use of the antibiotic streptomycin sulfate (SE) in the control of endogenous bacterias in micropropagated nodal segments of Eugenia involucrata. The treatments were: T1 (explants without previous contamination); T2 (explants with previous contamination); T3 (explants without contamination and immersed in SE solution at 100 mg L-1 for 5 min); T4 (contaminated explants and immersed in SE solution at 100 mg L-1 for 5 min); T5 (explants without contamination and inoculated in a medium containing 100 mg L-1 of SE); and T6 (contaminated explants and inoculated in medium containing 100 mg L-1 of SE). After 30 days of in vitro culture, there was no significant effect on the variables survival (mean 94.44%) and fungal contamination (mean 6.94%). The highest bacterial contamination was observed in T3 and T4 (100%), whereas in T5 (16.67%) the lowest average was observed. In T5 was observed the greatest number of leaves (mean 3.83), which differed statistically from T2 and T4, which presented the lowest averages. Immersion does not control the bacteria proliferation. Inoculation in the absence of prior contamination controls bacteria proliferation whereas inoculation with contaminated explants does not reduce it. Leaf development is hindered by the presence of bacteria.

Keywords : tissue culture; micropropagation; endogenous bacteria.

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