SciELO - Scientific Electronic Library Online

 
vol.20 issue2Negotiating process of the Interregional Framework Cooperation Agreement EU-MERCOSUR. 1995-2004: Major Challenges author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Investigación Agraria

On-line version ISSN 2305-0683

Abstract

PORTELA DE OLIVEIRA, Glauce; CLARETE CAMILI, Elisangela  and  MAGALHAES MORAIS, Otoniel. Methodology for tetrazolium in cowpea seeds. Investig. Agrar. [online]. 2018, vol.20, n.2, pp.110-117. ISSN 2305-0683.  https://doi.org/10.18004/investig.agrar.2018.diciembre.110-117.

The objective of this study was to determine the most efficient method for the evaluation of the physiological quality of cowpea (V. unguiculata) seeds by the tetrazolium test. We evaluated two seed lots of two cowpea cultivars, BRS Guariba and BRS Novaera. The tetrazolium test was performed for each batch of seeds and cultivating, which underwent tetrazolium solution at concentrations of 0.075%, 0.25% and 0.5% for 3-4 h in BOD incubator at 35°C in absence of light. The seeds were evaluated for uniformity and intensity of color presented by the tissues, being classified as: weak staining, suitable staining or strong staining. To evaluate the efficiency of the tetrazolium test, seeds of both cultivars and their lots were submitted to the germination and vigor tests. The mean values found in the tests were compared by Tukey test (p <0.05) and calculated the simple correlation coefficients of Pearson (r). It was observed that the tetrazolium is effective to classify the force of cowpea seeds under subsequent immersion in tetrazolium solution at a concentration of 0.075%, for a period 4 h at 35°C.

Keywords : Vigna unguiculata; viability; vigor.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf )