SciELO - Scientific Electronic Library Online

 
vol.17 número2Tratamiento alternativo de semillas de trigo para el control de hongos fitopatógenos¹Evaluación del territorio y determinación de un índice del valor catastral de la tierra en el Distrito de Atyra índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Investigación Agraria

versión On-line ISSN 2305-0683

Resumen

DALZOTTO¹, Carlos Alberto  y  LALLANA², Víctor Hugo. Effect of testa in vitro germination of Bipinnula pennicillata (Rchb. F.) Sisternas & Salazar (Orchidaceae). Investig. Agrar. [online]. 2015, vol.17, n.2, pp.116-121. ISSN 2305-0683.  https://doi.org/10.18004/investig.agrar.2015.diciembre.116-121.

determined by the tetrazolium viability determination method (Tz). The viability of seeds stored in cold conditions (4 °C) was evaluated at 24, 28, 32 and 35 months, through tetrazolium testing method (24 hours - 0.5% Tz).  In vitro planting was carried out using seeds preserved in refrigerator for 35 months, in a M and S medium at half the concentration. Seeds were disinfected before planting. Treatments (T) evaluated were T1 for whole seeds and T2 for cut seeds, obtained viability was 80%. Germination occurred 61 days after sowing (das). Maximum germination took place 76 days after sowing with 49% and 53% germination rate for T1 and T2 respectively, with no significant differences (p = 0.05) between treatments; showing that seed coat did not limit in vitro germination. Germination was significantly lower as compared with seed viability. Seed viability was high and it slightly decreased (8%) over a period of 35 months. Protocorms oxidation slightly affected germination rates in both treatments. It was concluded that seed coat does not limit asymbiotic seed germination under in vitro conditions, though it does when obtaining protocorms with apical bud and rhizoids (61 das), achieving  8% more than  whole seeds.

Palabras clave : Bipinnula pennicillata terrestrial orchids,; germination; axenic culture; viability..

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons