Abstract

DALZOTTO¹, Carlos Alberto  and  LALLANA², Víctor Hugo. Effect of testa in vitro germination of Bipinnula pennicillata (Rchb. F.) Sisternas & Salazar (Orchidaceae). Investig. Agrar. [online]. 2015, vol.17, n.2, pp.116-121. ISSN 2305-0683.  https://doi.org/10.18004/investig.agrar.2015.diciembre.116-121.

determined by the tetrazolium viability determination method (Tz). The viability of seeds stored in cold conditions (4 °C) was evaluated at 24, 28, 32 and 35 months, through tetrazolium testing method (24 hours - 0.5% Tz).  In vitro planting was carried out using seeds preserved in refrigerator for 35 months, in a M and S medium at half the concentration. Seeds were disinfected before planting. Treatments (T) evaluated were T1 for whole seeds and T2 for cut seeds, obtained viability was 80%. Germination occurred 61 days after sowing (das). Maximum germination took place 76 days after sowing with 49% and 53% germination rate for T1 and T2 respectively, with no significant differences (p = 0.05) between treatments; showing that seed coat did not limit in vitro germination. Germination was significantly lower as compared with seed viability. Seed viability was high and it slightly decreased (8%) over a period of 35 months. Protocorms oxidation slightly affected germination rates in both treatments. It was concluded that seed coat does not limit asymbiotic seed germination under in vitro conditions, though it does when obtaining protocorms with apical bud and rhizoids (61 das), achieving  8% more than  whole seeds.

Keywords : Bipinnula pennicillata terrestrial orchids,; germination; axenic culture; viability..

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