Abstract

RAMIREZ MONZON, Daisy et al. Differential Expression of the gene fla 14 in soybean seed tegument. Investig. Agrar. [online]. 2015, vol.17, n.2, pp.108-115. ISSN 2305-0683.  https://doi.org/10.18004/investig.agrar.2015.diciembre.108-115.

The seed coat has an important role related to seed resistance and deterioration. Therefore, it becomes necessary to understand the mechanisms of genes responsible for traits which can assist developing cultivars with better seed quality. In this sense, the analysis of the expression of fla 14 gene, encoding for the Arabinogalactan protein, which is directly related to cell wall synthesis in soybean seed coat, can contribute to the advancement of this study. Thus, the aim of this work was to validate primers and analyze fla 14 gene expression profile in four soybean genotypes, two of these with black seed coat (TP and IAC 222) and higher lignin content, and two others with yellow seed coat (CD 202 and Potência) and lower lignin content. The plant tissue (seed coat) was collected 50 days after anthesis. qRT-PCR technique was used for genotypes gene expression analysis,  through which four combinations of primersconstructed from soybean EST sequence models were tested. The validation results demonstrated the efficiency of FLA 14 p1 and FLA 14 p2 primers in gene expression studies. Regarding fla 14 gene, it was possible to notice that the expression profile of this gene is quantitatively superior in soybean genotypes with black coat and higher lignin content when comparing it with genotypes with yellow coat and lower lignin content.

Keywords : Glycine max L; Marr; qRT-PCR; black seed coat; Arabinogalactan.

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