Resumo

DELGADO GODOY, Magalí Lidia; SARUBBI ORUE¹, Humberto Jorge  e  GONZALEZ SEGNANA, Luis. Identification of viral diseases transmitted by bean (Vigna unguiculata (L.) Walp.) seeds. Investig. Agrar. [online]. 2014, vol.16, n.2, pp.93-98. ISSN 2305-0683.

The experiments were conducted at the Facultad de Ciencias Agrarias of the Universidad Nacional de Asunción, San Lorenzo, Paraguay. The implementation period was from January to April 2013, with the aim of detecting seed-borne viral disease of bean (Vigna unguiculata (L.) Walp.), by biological and serological tests. For this purpose samples were collected from six (6) varieties of seeds and plants of V. unguiculata infected plots located in the experimental field of the Instituto Paraguayo de Tecnología Agraria (IPTA), Chore, Department of San Pedro. It was performed the sowing of the seeds collected, San Francisco, Crema Ñu, Chore S-BR1 Poty, Negro, Crema Japonés, Moteado. The resulting plants with symptoms were subjected to ELISA test using specific antibodies to the virus Cowpea severe mosaic virus (CPSMV) and Cowpea aphid-borne mosaic virus (CABMV), respectively, so as to detect the transmission of the virus by seeds. Subsequently, from macerated leaf material samples that gave positive reactions in the test, mechanical inoculations were performed to three healthy plants indicator species: Chenopodium amaranticolor, Vigna unguiculata and Sesamum indicum. All varieties tested were positive to CABMV but only the Negro and Moteado varieties were positive to CpSMV. Transmission of CABMV through bean seeds of the variety San Francisco was detected, having a transmission rate of 6%.

Palavras-chave : Vigna unguiculata (L.) Walp.; CABMV,; CpSMV,; virus.

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