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Investigación Agraria
versión On-line ISSN 2305-0683
Resumen
FERREIRA AGUERO1,2, Marcos Arturo y OLIVEIRA JANEIRO NEVES¹, Pedro Manuel. Selectivity of Metarhizium anisopliae to Telenomus podisi (Hymenoptera: Scelionidae). Investig. Agrar. [online]. 2014, vol.16, n.1, pp.21-28. ISSN 2305-0683.
Selectivity of entomopathogenic fungus M. anisopliae to egg parasitoid T. podisi was evaluated. Three bioassays were realized: in the first, a suspension of M. anisopliae conidia was sprayed over sterilized eggs of Euschistus heros and exposed to parasitism, in the second, conidial suspensions were sprayed over parasitized eggs and in the third, the suspensions were sprayed over glass plates (IOBC/WPRS) and dried at ambient temperature, forming a layer of conidia for adult parasitoids contact. The control group was sprayed with sterile distillated water + Tween 80 (0.01%). To evaluate the percentage of parasitism, emergence and viability of parasitoids, the cards containing parasite eggs were introduced in plastic bags and observed using a stereoscopic microscope. The results indicate that M. anisopliae do not affect T. podisi viability and parasitism, but its emergence can be reduced 25.81%, if the fungus is sprayed on parasitized eggs. Possibly, the fungus penetrates the egg and infects the parasitoid at some stage. Nevertheless, emergence reduction compared to control was low. Therefore, M. anisopliae can be considered selective to T. podisi, and can be used jointly, separating at maximum the spraying of fungus and the releases of parasitoid.
Palabras clave : Euschistus heros; compatibility,; biological control,; entomophatogenic fungus,; eggs parasitoid..