Abstract

ALEX, Zichner Zorz¹; MAURA ISABEL, Díaz Lezcano¹; LUIS ROBERTO, González Segnana²  and  MIRTHA, Vera de Ortiz. Effect of activated carbon on the control of oxidation in nodal segments of Eucalyptus grandis Hill ex Maiden grown in vitro. Investig. Agrar. [online]. 2012, vol.14, n.2, pp.107-111. ISSN 2305-0683.

Clonal propagation by tissue culture of commercial interest woody spec ies, such as eucalyptus, represents a massive reproduction technique for genetically improved individuals with high demand. An experimental research was conducted at the Laboratorio de Biología of the Facultad de Ciencias Agrarias, Universidad Nacional de Asunción with the objective to establish a protocol for in vitro clonal propagation of Eucalyptus grandis Hill ex Maiden. The hypothesis was that the use of activated carbon controls phenolic oxidation of the nodal segments of in vitro propagated Eucalyptus grandis. The experimental design was completely randomized with 4 treatments and 5 replications. Treatments consisted of four concentrations (0, 1, 2 and 3 g/L) of activated carbon (AC) added to the composition of Murashige and Skoog salts. Hundred nodal segments were used evenly distributed between treatments. The variables measured every five days up to 30 days of incubation were percentage of contamination, oxidation, survival, sprouting, rooting and shoot length. Variables were analyzed for variance, means were compared using test of Tukey at 5%. The results showed 61% of cultures grown in vitro, highlighting the effect of the antibiotic Tetracycline on the mother plants, controlling contamination at 75% of the cultures. Activated carbon at the 3 g/L concentration, controlled 100% oxidation of the tissues. In conclusion, this protocol effectively enabled plant regeneration from nodal segments of Eucalyptus grandis Hill ex Maiden cultivated in vitro.

Keywords : Eucalyptus grandis Hill ex Maiden,; activated carbon,; micropropagation,; oxidation..

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