Abstract

ORTIZ R, N. Clostridium Botulinum EN MUESTRAS DE SUELO Y LODO DE LA REPÚBLICA DEL PARAGUAY. Compend. cienc. vet [online]. 2015, vol.5, n.1, pp.14-19. ISSN 2226-1761.  https://doi.org/10.18004/compend.cienc.vet.2015.05.01.14-19.

ABSTRACT. Soil and mud samples were collected for the study of the presence and distribution of Clostridium botulinum. A total of 54 samples from 12 departments of the country were examined, of these, 36 were collected from livestock farms, 9 from the Paraguay river, 6 from Ypacaraí lake, 3 from marshes. Samples were examined following the procedure described by Smith and Moryson (1975), inoculated in cooked meat medium and incubation at 30° C for 6 to 8 days; filtered supernatant from each sample was inoculated in mice to determine the presence of Clostridium botulinum toxins. Positive filtrates were subject to neutralization test using specific antitoxins, inoculated into mice for toxin typing. The presence of toxin was demonstrated in 18 (33%) of the total number of samples studied; toxin type A was detected in 1 (1.9%), type C in 6 (11.1%) and type D in 7 (13%) samples. The toxicity produced by the filtrates of 4 samples was weak to be typed by the toxin neutralization test in mice.

Keywords : Clostridium botulinum; toxin; bovine; soil; Paraguay.

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