Abstract

MONTES DE OCA, A et al. PROTECTIVE ACTION OF ADVENTICIOUS ON THE VASCULAR CALCIFICATION. Compend. cienc. vet [online]. 2013, vol.3, n.2, pp.26-32. ISSN 2226-1761.

ABSTRACT. The role of endothelium and the vascular Smooth Muscle Cells of the middle laye ron the formation of vascular calcifications (VC) has been study in deep. However, no attention was payed to the rol of adventicious in this process. Perhaps, the potential of this tissue was overlooked because the adventicious was consided hardly as a soft connective tissue, without a specific function. On the other hand, a published work (1) stresses a new paradigma VC of " From out to in”, in which the adventicious can participate as a forerunner in the answer to vascular lesion. The aim of the present study was to determine the roll of the adventicious in the development of VC in a in vitro model. For this porpose, aortic rings of wistar rats were cultured with adventicious (with/adv) and without (wout/adv) in culture media with normal levels of phosphorus (Control - P 0,9 mM) and elevated (P 3,3 mM). mineral content (Ca and P). Of the aortal rings were measured by colormetry and by mean of histological colored slide tissues by Von Kossa method. The adition of P 3,3 mM to the culture media resulted in an increase in mineral content of all the rings. The rings of the wout/adv treated with P 3,3 mM showed higher mineral content than those of w. adv group cultured under the same conditions. There were no differences in mineral content of both w. adv and wout adv groups cultured under normal concentration of P (0,9 mM). In conclusion, the adventicious play an protective roll on the VC in aortal rings exposed to high concentrations of in vitro.

Keywords : vascular calcifications; phosphorus, calcium; in vitro.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )