Abstract

ESCURRA, José; NOSEDA, Diego  and  FERREIRA, Francisco. Validation of an ethanol quantification method using the potassium permanganate reagent. Rep. cient. FACEN [online]. 2021, vol.12, n.2, pp.74-82. ISSN 2222-145X.  https://doi.org/10.18004/rcfacen.2021.12.2.74.

The determination of the ethanol concentration is an essential step in the fermentation processes, because depending on the progress of the fermentation process, they depend on specific steps in the optimization of the process. The potassium permanganate method is a quick, efficient and low-cost option that will allow monitoring the progress of ethanol production. In this work, the validation of this method of quantification of ethanol was carried out applying the Linearity, Precision and Veracity tests. For the first two trials glucose and ethanol standards were prepared, which were subjected to the reaction with DNS and potassium permanganate respectively. For the Veracity test, a known ethanol concentration was added to the YPD medium and pre-treated followed by DNS and Potassium Permanganate tests. The results indicate that this method yields accurate and reliable results, making it a viable alternative for the determination of ethanol concentration during a fermentation process.

Keywords : alcoholometry; colorimetry; industrial fermentation; potassium permanganate.

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