Abstract

LOPEZ BENITEZ, Yolanda Amelia; MOREIRA, Leandro Marcio  and  BENITEZ RODAS, Gilberto Antonio. Bacteria associated with Sarcocornia neei (Lag.) With apparent hydrolytic activity and biological control from the Paraguayan dry Chaco. Rep. cient. FACEN [online]. 2021, vol.12, n.1, pp.21-31. ISSN 2222-145X.  https://doi.org/10.18004/rcfacen.2021.12.1.21.

Halophyte plants show a perfect niche to isolate bacteria of interest, as they are adapted to extreme environments, these bacteria could be analyzed and exploited for their enzymatic capacities in research and in various industries. In this paper we analyze the enzymatic activity of endophyte bacteria and the rhizosphere of Sarcocornia neei, a halophyte that develops in the Chaco salt marshes. Enzyme activity was determined using Petri dishes, containing base medium, supplemented with 2 g / l (starch, carboxymethylcellulose, pectin and casein). Enzymatic degradation was determined by the formation of halos. A total of 83 bacterial isolates were obtained, 80 being Gram positive (96.3%) and 3 Gram negative (3.6%). In qualitative tests for the production of hydrolytic enzymes, producers of amylases 50.6%, celluloses 40.9%, pectinases 36.1% and proteases 10.8%. The bacterial isolates capable of producing hydrolytic enzymes were mainly endophyte bacteria, of which seven isolates were capable of simultaneously producing three hydrolytic enzymes amylases, celluloses and pectinases. Finally, the bacteria capable of inhibiting the growth of F. solani were 13.8%, while 4.6% of the isolates were capable of inhibiting the growth of K. pneumoniae and only 0.6% showed antimicrobial activity against S. aureus.

Keywords : Sarcocornia neei; halophytes; hydrolytic enzymes.

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