Abstract

MELGAREJO-TOUCHET, Nancy et al. Antimicrobial resistance in Escherichia coli from cecal samples of cattle for meat slaughtered in meat processing plants of the Mburicao stream area, Asuncion, Paraguay. 2021. Mem. Inst. Investig. Cienc. Salud [online]. 2022, vol.20, n.3, pp.51-59. ISSN 1812-9528.  https://doi.org/10.18004/mem.iics/1812-9528/2022.020.03.51.

Antimicrobial resistance is one of the most critical concerns worldwide since it determines an increase in patient morbidity and mortality and the increase in healthcare costs, a problem whose approach must be carried out under the One Health approach. The main objective of this work was to evaluate antimicrobial resistance in Escherichia coli from cecal samples of cattle for meat slaughtered in meat processing plants in the Arroyo Mburicao area, Asunción-Paraguay, from September to November 2021. One hundred eighty one samples of cattle from the Eastern and Western Region were collected, and cultured on chromogenic agar supplemented with ciprofloxacin, colistin, cefotaxime, and meropenem. Isolated colonies were identified, genotyped (conventional PCR) and subjected to susceptibility tests. The main finding was the confirmation of resistance to fluoroquinolones in 8.3% due to the carrying of genes Qnr S, Qnr B and aac-6'-Ib-cr, involved in the plasmid mechanism of resistance to quinolones. In addition, the accompanying resistance found in the isolates was 100% resistant to tetracycline and 53% to trimethoprim/sulfamethoxazole. Isolates with resistance to cefotaxime, colistin and meropenem were not found. The results obtained are highly relevant for the generation of knowledge about the resistance profiles of microorganisms in the veterinary sector. It can contribute to the development of national therapeutic guidelines for the prudent use of antimicrobials, and supporting multisectoral work in the fight to contain antimicrobial resistance.

Keywords : Antimicrobial resistance; cattle for meat; Escherichia coli; Mburicao stream; Paraguay.

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