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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528


PORTILLO, Carmen et al. Enteroviruses associated with Central Nervous System Infections confirmed by molecular biology. Mem. Inst. Investig. Cienc. Salud [online]. 2019, vol.17, n.1, pp.25-31. ISSN 1812-9528.

Approximately 90% of aseptic meningitis is caused by enterovirus (EV), a member of the Picornavirus family. EV is ubiquitous, spread by fecal-oral route and direct contact and responsible for outbreaks or sporadic cases with important morbidity. Diagnosis is based on clinical presentation, cerebrospinal fluid (CSF) analysis and polymerase chain reaction (PCR), the gold standard that substitutes viral isolation and serology. The objective of this descriptive, cross-sectional study was to identify EV by RT-PCR in the CSF of patients with clinical suspicion of aseptic meningitis, admitted to public and private medical centers of Asuncion and the Central Department of Paraguay from November 2007 to November 2014. Extraction of genetic material was carried out with Quiagen® DNA and RNA minikit which was subjected to a RT-PCR. CSF samples from 203 patients were included, 124 (61%) were children (range 4 days-15 years) and 79 (39%) adults (age range 16-81 years). Seventy seven (38%) were from public hospitals and 126 (62%) from private health centers; 115 (57%) were males. Enterovirus RNA was detected in 166 (82%) samples, 90 (54%) from children and 76 (46%) from adults. Most of the positive samples were collected from October to April. This study is the first in Paraguay and showed important involvement of EV in patients with infections of the NCS compatible with aseptic meningitis of viral etiology. Clinical suspect was higher in children, however, positive rate were greater in adults. Positive cases were more frequent in the hot season.

Keywords : enterovirus; meningitis; PCR; Paraguay.

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