Abstract

BOBADILLA, María Liz et al. Detection and typing of Human Papilloma Virus during a virological screening for detection of cervical lesions in Asunción, Paraguay. Mem. Inst. Investig. Cienc. Salud [online]. 2019, vol.17, n.1, pp.6-15. ISSN 1812-9528.  https://doi.org/10.18004/mem.iics/1812-9528/2019.017(01)06-015.

In Paraguay the incidence of cervical cancer (CC) is higher than those observed in other countries of the region. The etiological agent associated with CC is human papilloma virus (HPV), essentially oncogenic high-risk types. The objective was to describe epidemiological aspects of genital infection by high-risk human papilloma virus (HR-HPV) in women aged 25 to 64 years who consulted in services of Cervical Pathology of the MSPyBS, from May to December 2013. We used the Cobas 4800 HPV Test (Roche) that allows the individual detection of HPV-16 and HPV-18 and a pool of other HR-HPV including 12 high-risk genotypes. Other HR-HPV were typed by reverse line hybridization (RLB). Among the 495 women included in the study, 72 positive cases (14.5%) of HR-HPV were detected. We identified 19 viral types; the most frequent was HPV-16 (2.1%), followed by HPV-31, 33, 58 and 66; HPV-18 appears in sixth place. This work provides the first data on the implementation of molecular techniques for the detection and typing of HPV as part of the public health system in Paraguay. The predominance of HPV-16 confirms its wide circulation worldwide and, given its greater oncogenic potential, represents an alert to be considered, especially in women over 30 years of age who carry a persistent infection. These data support the importance of the sensible implementation and the appropriate use of the molecular tests currently available for the prevention and control of the CC.

Keywords : human papilloma virus; Cobas 4800 HPV-Test; genotyping; cervical cancer.

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