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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528

Abstract

GONZALEZ-ACHAR, Idelina et al. Antigenic profile in the acute and chronic phase of toxoplasmosis in pregnant women using the Western Blot technique. Mem. Inst. Investig. Cienc. Salud [online]. 2018, vol.16, n.3, pp.35-43. ISSN 1812-9528.  https://doi.org/10.18004/mem.iics/1812-9528/2018.016(03)35-043.

Toxoplasmosis is an endemic disease with variable global prevalence, being the causative agent the parasite Toxoplasma gondii. For an accurate diagnosis of a T. gondii infection, combinations of serological methods are required. Recent studies have reported that the Western Blot technique allows the detection of antigenic proteins as markers of infection, as well as certain protein profiles as possible indicators of acute and chronic phases of infection. The objective of the study was to identify the specific antigenic profile associated with different phases of toxoplasmosis. Fifty five sera from pregnant women with toxoplasmosis were included in the study, differentiated in acute and chronic phase of the disease by an IgG Avidity ELISA. By using the Western Blot method it was observed that antigenic proteins p35, p43, p45, p56 and p107 were recognized by 20-60% of sera from patients in acute phase, while p65, p95, p98 and p113 were recognized by 17-35% of sera from patients in chronic phase. It was observed that six antigenic proteins, p32, p38, p41, p48, p59 and p72, were recognized by more than 60% of sera from patients in both acute and chronic phases. The results obtained in this study suggest that these six proteins could be considered as diagnostic markers of the disease.

Keywords : Toxoplasma gondii; Western Blot; antigenic profile.

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