Abstract

DEL PUERTO, Florencia et al. Molecular surveillance of malaria in potential wild reservoirs that live in a historically endemic region of Paraguay. Mem. Inst. Investig. Cienc. Salud [online]. 2018, vol.16, n.3, pp.30-34. ISSN 1812-9528.  https://doi.org/10.18004/mem.iics/1812-9528/2018.016(03)30-034.

In Paraguay, autochthonous cases of malaria have not been recorded since 2011. A cross-sectional observational descriptive study was conducted in 6 monkeys and 23 birds living in a historically endemic region of Paraguay to identify wild reservoirs of plasmodium parasites that cause malaria. DNA was extracted by the Chelex method from a blood drop in a filter paper, and parasite detection was performed by the seminested multiplex PCR. By this method, parasites were not detected in any of the 29 samples. The risk of potential sylvatic circulation of the parasites causing malaria was evaluated. Considering the presence of anopheline mosquitoes in the area, the fact that we did not find any positive cases is a good indicator as our country was recently certified as a malaria-free country by the WHO.

Keywords : wild reservoirs; malaria; Paraguay.

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