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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528


BERNAL AGUIRRE, Laura Elena et al. Optimization of a conventional PCR technique for detection of human papillomavirus type 16 and 18. Mem. Inst. Investig. Cienc. Salud [online]. 2018, vol.16, n.3, pp.6-12. ISSN 1812-9528.

Cervical cancer is the second most common female cancer worldwide. It is caused by the human papilloma virus (HPV). Thirteen genotypes of high oncogenic risk human papilloma viruses (HPV-HR) have been identified, among which types 16 and 18 are the most frequently detected in cervical cancer. In Paraguay, they are detected in 70% of the invasive cancer cases. Therefore, the objective was to standardize and determine the detection limit of a conventional PCR technique for the detection of HPV 16 and 18. Better results were observed with 2mM MgCl2 and 60°C for the alignment temperature in detection of HPV 16 and 18 DNA. The limit of detection was 14.6x10-11ng/μL for HPV 16 and 21.7x10-12ng/μL for HPV 18. This work will help other studies for the detection and identification of these viral types by PCR in order to identify a group of HPV-HR positive women who have higher risk for the development of lesions and cervical cancer and need a closer follow-up.

Keywords : Cervical cancer; HPV 16; HPV 18; PCR.

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