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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528

Abstract

DEL PUERTO, Florencia et al. Sex identification by molecular methods in 9 species of birds from the wild animal research center of ITAIPU dam on the paraguayan side. Mem. Inst. Investig. Cienc. Salud [online]. 2017, vol.15, n.3, pp.89-92. ISSN 1812-9528.  https://doi.org/10.18004/mem.iics/1812-9528/2017.015(03)89-092.

In monomorphic species living in captivity, determining the sex is important because it may allow choosing mating strategies to enhance reproduction. Sex can be determined either by non-molecular recognition methods, which are only feasible in adult birds, or by molecular techniques, which may allow sexing monomorphic species at young ages. Thus, in this study, we used molecular techniques such PCR to amplifying the CHD gene by using 2550F/2718R primers. This method allowed us to determine the sex in 19 out of 23 bird specimens from the Centro de Investigación de Animales Silvestres (CIASI) of the ITAIPÚ BINACIONAL at Paraguayan side. The specimens belonged to the following species: Amazona aestiva (3), Pipile grayi (1), Bubo virginianus (1), Ara chloropterus (4), Crax fasciolata (6), Caracara plancus (2), Chauna torquata (3), Cariama cristata (1) and Cairina moschata (2). The 2550F/2718R primers were useful for determine the sex of the studied species.

Keywords : Sexing; birds; 2550F/2718R; Paraguay.

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