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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528

Abstract

MARTINEZ-PAVETTI, Ana María et al. Antigenic profile characterization of soluble protein extracts from two Trypanosoma cruzi strains. Mem. Inst. Investig. Cienc. Salud [online]. 2017, vol.15, n.3, pp.27-34. ISSN 1812-9528.  https://doi.org/10.18004/mem.iics/1812-9528/2017.015(03)27-034.

The parasite Trypanosoma cruzi is the causal agent of Chagas disease.It presents a wide biological and genetic diversity, therefore it is grouped into 6 taxonomic units..The strains Y, CL Brener and one strain isolated in Paraguaywere used in this study in order to determine their protein and antigenic profiles, applying techniques such as SDS-PAGE, ELISA and Western blot. Similarities were observed in the protein prifles of the soluble extracts of Lot 73 Y and Lot 74 Y both obtained from the same strain, and the different profile of these with those of CL Brener and Py strains. Protein bands of common molecular weights, manly those consideredlow, were detected between extracts. By testing the four extracts against chagasic and non-chagasic sera, positive and negative controls, the antigenic capacity was verified and the importance of utilizing different test to confirm diagnosis was shown. In addition antigenic proteins common to the three extracts used in the Western blot were detected and can be further investigated due to their antigenic potential, an interesting characteristic for diagnostic purpose.

Keywords : Trypanosoma cruzi; western blot; Chagas disease.

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