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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528

Abstract

OPHELIE, Clotilde  and  MOLIN QUESTE, Marie. Phenotypic detection of Carbapenemases in P. aeruginosa in patients attending the Hospital de Clinicas in San Lorenzo, from February to July 2013. Mem. Inst. Investig. Cienc. Salud [online]. 2016, vol.14, n.1, pp.25-31. ISSN 1812-9528.  https://doi.org/10.18004/Mem.iics/1812-9528/2016.014(01)25-031.

P. aeruginosa is one of the most important opportunistic pathogens that cause infections with high morbidity and mortality. Carbapenems are antibiotics with a broad spectrum of activity and highly powerful. This makes them indispensable in the empirical treatment. P. aeruginosa has various mechanisms of resistance, including metallobetalactamase (MBL) type carbapenemases Due to the numerous reporst of MBL producing bacteria, it is important to apply simple tests that are practical and inexpensive as routine protocol in order to rapidly identify MBL producing bacteria. The objective of this study was to determine the presence of phenotypically carbapenemases in isolates of P. aeruginosa. A prospective, descriptive cross- sectional study was performed in isolates of P. aeruginosa from patients attending the Hospital de Clinicas - San Lorenzo from February to July, 2013. Two hundred thirty two isolates of P. aeruginosa were studied. Those isolates suspicious of having Carbapenemases were subjected to two phenotypic detection methods: discs of EDTA and discs of dipicolinic acid - Meropenem (DPA-ME). Of these isolates, 30 were synergistic with the technique of EDTA and 18 positively isolated with DPA discs. Through these phenotypic methods the presence of strains producing MBL type carbapenemases was determined at a frequency of 7.8%. The disc combination tests might be very useful in the daily practice to provide fast and reliable detection of MBL carbapenemases in P. aeruginosa isolates, where molecular biology tests are not available.

Keywords : Carbapenemases; MBL; dipicolinic acid; P. aeruginosa.

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