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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528


SANABRIA, R et al. Anti-fungal susceptibility profile of Candida spp isolates by the microdilution method. New cutoff points for fluconazole. Mem. Inst. Investig. Cienc. Salud [online]. 2014, vol.12, n.1, pp.33-40. ISSN 1812-9528.

Yeast infections are very important because of their relatively high frequency and increased resistance to antifungal agents, especially to azole derivatives, and in particular, to fluconazole. The objective of this study was to determine the minimum inhibitory concentration (MIC) of fluconazole and amphotericin B in Candida isolates in clinically significant samples from hospitalized patients in Paraguay from May 2010 to August 2011 using the old and new criteria for fluconazole. The MICs of the antifungals were determined by the microdilution method (M27 A-2) according to CLSI recommendations. Fifty isolates were evaluated, being C. albicans the predominant species (40%) followed by C. parapsilosis and C. glabrata in equal proportion (20%), C. tropicalis (16%) and C. krusei (4%). Using the old cutoff, five isolates were resistant to fluconazole (10%), two naturally resistant isolates (C. krusei), two C. tropicalisand one C. albicans. Three C. glabrata and one isolates of each species of C. tropicales, C. parasilopsis y C. glabrata showed sensitivity dose dependent (SDD). With the new cutoff, the number of resistant isolates increased to seven (14%), of which two were SDD based on the old criteria. Nine C. glabrata and two C. albicans,previously classified as sensitive, changedto SDD. All isolates were susceptible to amphotericin B. With the new cutoff points, important variations were observed in the isolates susceptibility profiles, particularly in C. glabrata, supporting the need of species identification, permanent surveillance of the isolates resistance to anti-fungals and correlation of the sensitivity results with the clinical response to treatment.

Keywords : fluconazole; amphotericin B; Candida spp.

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