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Memorias del Instituto de Investigaciones en Ciencias de la Salud

On-line version ISSN 1812-9528


GIMENEZ, G et al. A rapid and economic option for human papillomavirus detection by PCR in cervical samples with denaturing reagent. Mem. Inst. Investig. Cienc. Salud [online]. 2014, vol.12, n.1, pp.07-13. ISSN 1812-9528.

Studies have demonstrated the usefulness of the hybrid capture II (CH IIâ) in the detection of oncogenic high risk human papillomavirus (HR-HPV) as a primary screening method for detection of cervical cancer, as well as the benefits of PCR that allows access to viral typing methods. The objective was to detect HPV by PCR from cervical samples processed by CH IIâ. It was a cross-sectional study including 141 cervical samples of women attending the IICS, UNA. The samples were processed by CH IIâ and stored with denaturing reagent at -80ºC. Then, they were diluted 100 times with distilled water and subsequently processed by PCR. HPV was detected in 51% and 43% of the samples analyzed by CH IIâ and PCR respectively. Seventeen of 23 positive samples by CH IIâ with relatively low viral load were negative by PCR. This could be due to degradation of the material. In addition, six negative samples by CH IIâ were positive by PCR suggesting the presence of infection with HPV types not included in CH IIâ. These results suggest that it is possible to detect HPV by PCR from samples processed by CH IIâ prior dilution. This is a quick, easy and economic alternative which minimizes the risk of losing the genetic material in the extraction process, and allows access to viral typing methods that could provide data about circulating HPV types to carry out surveillance in the post- vaccine era.

Keywords : HPV detection; cervical sample; denaturing reagent; hybrid capture II â, PCR.

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