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Memorias del Instituto de Investigaciones en Ciencias de la Salud

versão On-line ISSN 1812-9528

Resumo

SANCHEZ, Z  e  RUSSOMANDO, G. Molecular identification of Trypanosoma cruzi lineages and sub lineages in congenitally infected children from endemic areas of Paraguay. Mem. Inst. Investig. Cienc. Salud [online]. 2012, vol.10, n.1, pp.56-61. ISSN 1812-9528.

American trypanosomiasis is an infection caused by the protozoan flagellate Trypanosoma cruzi that is spread throughout the Americas. T. cruzi is a heterogeneous species comprising several sub-populations circulating in domestic and wild vertebrate and invertebrate hosts. This genetic diversity has been detected with molecular techniques showing that there are major clones circulating in several hosts and different geographic areas of Latin America. T. cruzi isolates have been divided into two genetic groups named by an international consensus as T. cruzi I (lineage 2-associated with enzootic cycle) and T. cruzi II (lineage 1-associated with the domestic cycle). The interaction of infective clones of T. cruzi and human host could determine the morbidity of the disease. In this work, we used PCR technique to amplify constitutive genes of T. cruzi, 24Sa ribosomal gene and the mini exon (ME) gen. Lineages of T. cruzi were detected in 112 samples (83%) out of 135 samples of infected children from two Departments of Paraguay that are endemic for Chagas disease (Cordillera and Paraguarí). Regarding their origin, 53 samples were from Paraguarí and 59 samples from Cordillera. The sub-lineages found in Paraguarí were: 34TcIIc, 17 TcIId, 1 TcIIb-IIe and 1 ND sample (not determined). In Cordillera: 37 TcIIc, 19 TcIId, 1 TcIIb-IIe and 2 ND samples. Wild lineage I was not found in any of the two areas studied.

Palavras-chave : Trypanosoma cruzi; lineages; vector.

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