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Memorias del Instituto de Investigaciones en Ciencias de la Salud

versão On-line ISSN 1812-9528


ACOSTA, ME et al. Concordance between the IgG avidity ELISA tests developed at the Instituto de Investigaciones en Ciencias de la Salud and a commercial avidity ELISA. Mem. Inst. Investig. Cienc. Salud [online]. 2010, vol.8, n.2, pp. 39-43. ISSN 1812-9528.

ABSTRACT The acute infection caused by Toxoplasma gondii in pregnant women is mostly asymptomatic and detectable only by antibodies that could severely affect the fetus if the infection is not diagnosed and treated precociously. In this cross-sectional observational, the analytical the IgG avidity ELISA test for toxoplasmosis, developed at the Instituto de Investigaciones en Ciencias de la Salud (IICS), was compared to a commercial avidity kit InmunoLISA (Organics, USA). For the concordance, 41 serum samples kept at -20ºC at the Department of Production-Biochemistry of IICS were tested. The concordance obtained was 92.7% and a Kappa index of 0.820 with IC95% (0.6-1) and p <0.0001. The low avidity index suggests an acute infection but for diagnosis this result should be accompanied by other serologic tests and clinical symptoms. Instead, a high avidity index suggests a chronic infection.

Palavras-chave : Toxoplama gondii; avidity; diagnosis.

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