Memorias del Instituto de Investigaciones en Ciencias de la Salud
versão On-line ISSN 1812-9528
ROIG, C et al. Maintenance and titration of dengue virus for the obtainment of viral antigens. Mem. Inst. Investig. Cienc. Salud [online]. 2009, vol.7, n.1, pp.61-65. ISSN 1812-9528.
Dengue is a serious public health problem that has neither vaccine nor specific treatment. Serology is the most frequently used diagnosis method, specifically the anti-dengue IgM detection. The viral antigens employed in this method could be prepared from Aedes albopictus cell cultures (C6/36). The objective of this study was to maintain the four viral serotypes (D1 (RIO), D2 (RIO), D3 (H-87), D4 (BV)) on C6/36 cells for the preparation of viral antigen in the future. The C6/36 cells were cultured in L-15 medium supplemented with 10% FCS, infected with 50 µl of each viral serotype and then incubated for 5-7 days at 28ºC. After confirmation of the infection by indirect immunofluorescence (IIF), viral titration was performed by lysis plaque assay. The serotypes titres obtained were as follows: [2.9 x 106 PFU/ml] for D1 (RIO), (4.4 x107 PFU/ml) for D2 (RIO), (6.4 x 10 7 PFU/ml) for D3 (H87) and (5.1 x106 PFU/ml) for D4 (BV). The production of viral antigens is very important because they could be used in several diagnosis methods.
Palavras-chave : Dengue; cell culture; antigen.