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Pediatría (Asunción)

On-line version ISSN 1683-9803


CUASTUMAL, Maria Eugenia; RODRIGUEZ, Maria Isabel; MEJIA RIVERA, Luis Fernando  and  GOMEZ URREGO, José. Real-time multiplex PCR for sepsis (Film array): characterization as a diagnostic method in a pediatric center in Cali. Pediatr. (Asunción) [online]. 2020, vol.47, n.2, pp.69-73. ISSN 1683-9803.


Sepsis is a condition that generates high morbidity and mortality: The early detection of the causative microorganisms is essential for timely management.


to determine the frequency of detection and typing of microorganisms by multiplex PCR (Film Array) in pediatric patients (under 18 years of age) admitted to the emergency department with a confirmed diagnosis of sepsis.

Materials and Methods:

This was a retrospective, descriptive and observational study, Positive blood culture panels of patients under 18 years of age, diagnosed with sepsis, were analyzed using multiplex PCR technology (Film Array) at the Hospital Clínica Infantil Club Noel. The results were compared. The data was analyzed with Microsoft Excel, using descriptive statistics.


Of 101 positive blood cultures, the Film Array molecular panel detected 92 (91%). There was a predominance of sepsis from a respiratory source and children under 3 years of age. The most frequently detected germs were gram-negative organisms, both in the blood culture and in the molecular panel.


the findings regarding the detection of bacteremia in our population, using multiplex real-time PCR, demonstrate its detection ability and its importance in early diagnosis and timely treatment.

Keywords : Bacteremia; sepsis; pediatrics; polymerase chain reaction; diagnosis..

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