SciELO - Scientific Electronic Library Online

vol.46 issue1High-Frequency Ventilation in Newborns. A Needed Capability in Neonatal Units author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Pediatría (Asunción)

On-line version ISSN 1683-9803


PORTILLO, Carmen; SAMUDIO, Gloria; ORTIZ, Lidia  and  RAMOS, Pasionaria. Detection of Cytomegalovirus using real-time PCR in the blood of younger infants in Paraguay 2015-2017. Pediatr. (Asunción) [online]. 2019, vol.46, n.1, pp.26-32. ISSN 1683-9803.


Cytomegalovirus (CMV) infection is frequent during the first years of life in underdeveloped countries and in lower socioeconomic groups. Objective: to determine the prevalence of CMV infection identifying the viral genome of Cytomegalovirus by using PCR in blood samples from infants from 0 to 12 months of age who were hospitalized in the intensive care unit with a suspected infection.

Materials and methods:

This was a retrospective, descriptive, observational and cross-sectional study. From January 2015 to May 2017, we analyzed peripheral blood samples from infants with suspected CMV infection. Promega® reagents were used, followed by real-time PCR with Rotor Gene® equipment. Variables were: age, sex, place of origin, clinical symptoms and results of the polymerase chain reaction. The data was analyzed using descriptive and analytical statistics.


146 real-time PCR results from blood samples were included, 84% (123/146) were infants under 6 months of age. In 42% (62/146) viral DNA was detected by PCR, of these, 38% were girls; 28% were infants under 2 months of age. 17.7% (11/62) were preterm infants. Clinical symptoms and findings in the positive cases were prematurity, anemia in 8% Jaundice in 5.6%, respiratory difficulty in 5.4%, pneumonia in and 1.3%, and hepatosplenomegaly in 4%.


CMV infection was confirmed by real-time PCR in 42% of infants, 28% were between 0 to 2 months of age and 17.7% were preterm infants, similar to other studies.

Keywords : Cytomegalovirus; polymerase chain reaction; infants; congenital infection.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )