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Pediatría (Asunción)

On-line version ISSN 1683-9803


SALINAS, Claudia et al. Evaluation of the biofilm-forming capacity of methicillin-resistant strains of S. aureus that infected Paraguayan children. Pediatr. (Asunción) [online]. 2017, vol.44, n.3, pp.233-238. ISSN 1683-9803.


The capacity of S. aureus to adhere to surfaces and other bacterial cells by forming a biofilm is one of the main causes of the persistence of infections by this pathogen.


To analyze the capacity of S. aureus strains to form biofilm, using in vitro polystyrene plate culture techniques.

Materials and methods:

This was a descriptive, cross-sectional study. We studied and performed molecular characterization of viruence factors in ten strains of S. aureus, which were representative of the clonal groups detected in a total of 347 strains that caused skin and soft tissue invasive infections in children in 2012. The standardization of the in vitro assay was successful, allowing to classify the strains into 3 categories according to the intensity of biofilm formation.


100% of the analyzed strains were biofilm formers and were categorized as moderate formers.


The detection of the formation of bacterial biofilms, especially in chronic infections, is crucial, since it affects the choice of special strategies, such as surgical removal, for their elimination, given the ineffectiveness of traditional antibiotic therapies.

Keywords : S. aureus; biofilm; children.

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