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Memorias del Instituto de Investigaciones en Ciencias de la Salud
On-line version ISSN 1812-9528
Abstract
ABENTE, Sonia et al. Frequency of methicillin-resistant Staphylococcus aureus and PVL virulence factor in ambulatory patients with skin and soft tissue infections of Asuncion, Paraguay. Mem. Inst. Investig. Cienc. Salud [online]. 2016, vol.14, n.2, pp.8-16. ISSN 1812-9528. https://doi.org/10.18004/Mem.iics/1812-9528/2016.014(02)08-016.
Staphylococcus aureus (S. aureus) is currently the most common etiologic agent of skin and soft tissue infections. The isolation of methicillin-resistant S. aureus (MRSA) from infections of patients in the community has increased, becoming a public health problem worldwide. In Paraguay, there are few records about methicillin resistance and virulence factors at community level. Therefore, this descriptive observational study was performed to determine the frequency of MRSA and factor virulence of Panton-Valentine leukocidin (PVL) as well as the antimicrobial resistance profile accompanying methicillin resistance in S. aureus isolated from skin and soft tissue infections in ambulatory patients from two laboratories of Asuncion, Paraguay from October, 2012 to February, 2014. The bacterial identification was performed using conventional microbiological techniques and the antimicrobial susceptibility was determined by disk diffusion. The mecA and luk-PV genes were detected by PCR technique. Out of the 70 S. aureus isolates studied, 54.3% (38/70) was SAMR by phenotypic and molecular methods. PVL frequency was 15.7% (11/70) being higher in MRSA (21%; 8/38) than in the SAMS (9.4%; 3/32), 2.6% of the MRSA was resistant to ciprofloxacin and multidrug resistance was not observed in any isolates.A high frequency of MRSA was found compared with previous reports in Paraguay. It is required to strengthen surveillance, prevention and control of bacterial resistance strategies in hospital and community environments.
Keywords : methicillin-resistant Staphylococccus aureus; Panton Valentine leucocidin; mecA; skin and soft tissue infections.